Coding
katG

Part:BBa_K137068:Design

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-07-31)


katG +LAA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 228
    Illegal BglII site found at 1163
    Illegal BglII site found at 1290
    Illegal BamHI site found at 1080
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site directed mutagenesis to remove an EcoRI site.


Source

Genomic PCR off of DH10B

References